Cross-resistance Relationships between Paromomycin (aminosidine), Lividomycin, Kanamycin, and Capreomycin Resistances of Mycobacterium Tuberculosis

Introduction Paromomycin1•`4) and aminosidineo are aminoglucoside antibiotics recently discovered. Identity of paromomycin and aminosidine has been reportedo. Knowledge of cross-resistance-relationships in respect to this antibiotic is very scanty. ARCAMONE et al. 5) stated that a neomycin-resistant strain of Staphylococcus aureus was resistant to paromomycin. The present study was carried out in an aid to know cross-resistance-relationships of Mycobacterium tuberculosis in respect to this antibiotic and other aminoglucoside antibiotics and capreomycin. Methods Mycobacterium tuberculosis, H37Rv and Aoyama-B and eleven strains freshly isolated from patients, were used. The latter eleven strains were isolated from tuberculous patients previously not treated with any antituberculous agent by single colonyisolation technique. Tests for the resistance to various antituberculous agents were carried out by the "actual count" method"), and the degree of resistance was expressed as the highest concentration of an agent on which a small inoculum consisting of 20 to 100 viable units could form visible colonies after incubation at 37°C for 4 weeks. OGAWA egg medium was used throughout. The composition of the OGAWA egg medium is as follows : Basic solution (1% KH2PO4 and 1% sodium